Coding

Part:BBa_K4343099

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


FmDes12

FmDes12 encodes ∆-12 desaturase from Fusarium moniliform which functions in the desaturase and elongase pathway and could convert C18:1 to C18:2.

Description

The FmDes12 encodes a protein of 477 amino acids. Heterologous expression of FmDes12 could catalyze C18:1 to C18:2 in Y. lipolytica.

Literature

The enzyme has been studied for improving the ratio of ω-3 to ω-6 fatty acids in microbes and plants.

Reference

Damude, H.G. et al. Identification of bifunctional ∆12/ω3 fatty acid desaturases for improving the ratio of ω3 to ω6 fatty acids in microbes and plants. Proc. Natl. Acad. Sci. USA 103, 9446–9451 (2006).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 683
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1451


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Parameters
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